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. 2020 Mar 25;3:144. doi: 10.1038/s42003-020-0870-z

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a In situ hybridization with NHEJ1 and IHH in sections of the head region and neural tube of the wild-type E3 embryos and the autopod in the hindlimb of the wild-type E6 embryos. Sections are oriented with the rostral side up and the caudal side down in the head region; the dorsal side up and the ventral side down for the neural tube; and the anterior side up and the posterior side down for the autopod. Magenta arrowheads indicate gene expression. AS, antisense probe; S, sense probe. Bar, 100 μm. b Expression of IHH and ALAD in the wild-type embryos at stage 14, indicated by in situ hybridization. Images are oriented with the rostral side up and the caudal side down. Bar, 1 mm. c Neighboring sections of the extraembryonic blood vessels of embryos at stage 14. Expression of ALAD, IHH, and NHEJ1 was examined by in situ hybridization. Bar, 200 μm.