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. 2020 Mar 25;3:142. doi: 10.1038/s42003-020-0874-8

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a Schematic of R-iSAT. Two template DNAs encoding 16S rRNA and sfGFP are added into the PURE system. Recombinant ribosomal proteins (recombinant rps) bind to the transcribed 16S rRNA, and they are assembled into 30S subunits that interact with native 50S subunits to form the ribosome. Transcribed sfGFP mRNA is translated on the ribosome to synthesize sfGFP synthesis. b Time-course analysis of sfGFP synthesis in reactions without coupling of 16S rRNA transcription. c Time-course analysis of sfGFP synthesis in reactions with coupling of 16S rRNA transcription. R-rps represents recombinant ribosomal proteins, and IVT 16S rRNA represents in vitro transcribed 16S rRNA. Fluorescence intensities after subtracting the background intensity are shown. Error bars indicate standard deviation of triplicate measurements.