Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Mar 25;11:1559. doi: 10.1038/s41467-020-15267-z

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 3 — a, b Microglial morphology following a 60-min incubation in glucose-containing aCSF (a, 10 mM glucose) or aglycemic aCSF (b, 0 mM glucose) (for panel a–g, glucose aCSF: n = 7 mice, 14 slices, 307 cells; aglycemia: n = 7 mice, 14 slices, 322 cells). c, d 3DMorph rendering of representative microglial cells following a 60-min incubation in glucose-containing aCSF (c) or aglycemic aCSF (d). e Ramification index of microglia 60 min after control or aglycemia incubation. f Number of branch points per microglia. g Percentage of brain volume occupied by microglia. h Process motility of microglia following one hour of aglycemia. Shown are overlaid images obtained 10 min apart to show dynamic microglial motility (for h–j, n = 7 slices for glucose, 15 slices for aglycemia). i Quantification of microglial motility index over time during aglycemia treatment. j Microglial motility index after 60-min incubations in glucose-containing aCSF or aglycemic aCSF. Shown here is the mean microglial motility for a 15-min imaging period following the incubation time (*p = 0.0319, unpaired, two-tailed t-test). k, l Microglial response to a laser-induced lesion following a 60-min incubation in glucose-containing aCSF (k) or aglycemic aCSF (l) (for k–n, n = 5 slices for glucose, 7 slices for aglycemia). m Quantification over time of the change in fluorescence intensity of the area around the lesion (not including the lesion). n Percentage of microglia surrounding the lesion (within a 75 µm radius of the lesion) with processes reaching the lesion within 20 min (n = 6 slices for glucose, 11 slices for aglycemia). For e–g, n, NS, p > 0.05, unpaired, two-tailed t-test; for m, treatment factor is NS, p > 0.05, but time factor is ****p < 0.0001, two-way ANOVA; data are represented as mean ± SEM. Scale bars, 100 μm (a, b, left panels); 20 μm (a, b, right panels); 25 μm h; 50 μm k, l. Source data are provided as a Source Data file. See also Supplementary Fig. 2.