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. 2020 Mar 25;10:5386. doi: 10.1038/s41598-020-62190-w

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Cultured human umbilical endothelial cells (HUVEC) were exposed to different concentrations of visfatin/eNampt (0, 25, 50, and 100 ng/mL) for 18 h and the following proteins were determined by Western blot. Phosphorylated subunit of p65 (P-p65) and total p65 subunit, taking the ratio of Pp65 to p65 as a measurement of NF-κB activity (A); NLRP3 (B); the ratio of cle-caspase-1 to pro-caspase-1 (C); pro-IL-1β (D); and mature IL-1β (E). Data are expressed as the percentage (mean ± SEM) of the respective protein level in basal conditions. Protein levels were normalized by β-actin levels used as loading control. Every column includes at least 6 different experiments. *p < 0.05 vs respective basal values in the absence of visfatin/eNampt.