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. 2020 Mar 25;10:5386. doi: 10.1038/s41598-020-62190-w

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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In cultured HUVEC, the presence of the inflammasome related apoptosis-associated speck like protein containing a caspase recruitment domain (ASC-specks) was studied by immunofluorescence, either in control conditions or in the presence of 100 ng/mL visfatin/eNampt and/or 10 µmol/L FK 866, 1 µmol/L CLI 095, and 1 µmol/L MCC 950. Nuclei were counterstained with DAPI (blue) (630×). Arrowheads point ASC-specks aggregates in the different treatments.