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. 2020 Mar 25;10:5386. doi: 10.1038/s41598-020-62190-w

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(A) ASC-specks present in HUVECs exposed to 100 ng/mL visfatin/eNampt, alone or with 10 µmo/L FK 866, 1 µmol/L CLI 095, or 1 µmol/L MCC 950. (B). ELISA-measured IL-6 levels in the medium from cultured HUVEC submitted to 100 ng/mL visfatin/eNampt, alone or with FK 866, CLI 095 and MCC 950. (C) NLRP3 and (D) pro-IL-1β levels in cultured HUVEC exposed to 50 ng/mL visfatin/eNampt, alone or with 10 µmol/L FK 866, 1 µmol/L CLI 095, or 1 µmol/L MCC 950. Data (mean ± SEM) are expressed as the percentage of the respective values obtained in the presence of visfatin/eNampt. Protein levels were normalized by β-actin levels used as loading control. Every column includes at least 6 different experiments. *p < 0.05 vs basal; #p < 0.05 vs visfatin/eNampt.