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. 2020 Mar 25;10:5386. doi: 10.1038/s41598-020-62190-w

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Aortic wall or kidney homogenates from C57BL/6 mice infused for 7 days with saline solution or visfatin/eNampt (100 ng/kg/day) were employed to determine by Western blot the tissular aortic expression of P-p65 and p65 (A), NLRP3 (B), as well as the tissular renal levels of P-p65, p65 (C), NLPR3 (D), and neutrophil gelatinase-associated lipocallin (NGAL) (E). Data (mean ± SEM) are expressed as the percentage of the respective protein level obtained for a pool of saline-infused aortic homogenates, termed standard. Protein levels were normalized by β-actin levels used as loading control. Every column includes at least 4 different animals. *p < 0.05 vs standard.