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. 2020 Mar 25;10:5479. doi: 10.1038/s41598-020-62163-z

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Golgi like stained cells in the mPOA of pregnant females are neurons. (a) Golgi like stained neurons in mPOA at GD18. Soma, dendrites (arrows), as well as axon initial segment (arrowheads) shows WFA associated DAB staining; (b) Confocal images z-stack of fluorescent labeled WFA preparations, showed that PNNs in the mPOA are organized in a “honeycomb” structure as described for neocortical PNNs together with a distributed punctuated pattern; (c) mPOA semithin sections at GD18 confirm the neuronal identity and show the external surrounding distribution of the WFA associated DAB staining. Positive surface stained cells show neuronal features, including big heterochromatic nuclei with prominent nucleolus, and abundant cytoplasm containing Nissl bodies. Interestingly, some neurons are not stained indicating that PNNs positive cells correspond to a neuronal subpopulation within this mPOA region.