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. 2020 Mar 25;10:5479. doi: 10.1038/s41598-020-62163-z

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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PNNs expression in the mPOA during gestation. Panels (a–e) show representative slices from anterior to posterior mPOA of GD21 female brain. PNNs were evidenced using WFA staining. Notice the appearance of PNNs expressing neurons and its antero-posterior pattern distribution, with no WFA signal in the anterior (a,b), moderate in the medial (c), and high in the posterior region (d,e). Numbers in each panel indicate the distance caudal to bregma in millimeters.