Table 1.
Antibodies.
| Antibody | Source | Dilution | Catalog number (#) |
|---|---|---|---|
| Akt1/2 Ser473/474 | Cell Signaling Technology | 1:1000, 2% skim milk | 4051 |
| Akt2 | Cell Signaling Technology | 1:1000, 2% skim milk | 3063 |
| TBC1D4 Thr642 | Cell Signaling Technology | 1:1000, 2% skim milk | 4288 |
| HK II | Cell Signaling Technology | 1:1000, 2% skim milk | 2867 |
| GLUT4 | Thermo Fisher Scientific | 1:1000, 2% skim milk | PA1-1065 |
| TBC1D4 | Abcam | 1:1000, 2% skim milk | Ab189890 |
| OXPHOS | Abcam | 1:500, 2% skim milk | Ab110413 |
| UCP1 | Abcam | 1:1000, 2% skim milk | Ab10983 |
| Pyruvate Dehydrogenase | Grahame Hardie, University of Dundee, UK | 1:1000, 2% skim milk |