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. 2020 Mar 19;11:300. doi: 10.3389/fphar.2020.00300

Figure 3.

Figure 3

SAL treatment restored gut architecture and intestinal epithelial cell connections in ZDF rats. (A) H&E staining of rat ileum (original magnification: ×200; scale bar: 50 μm). (B) EM details of ileum structure in various groups (original magnification: ×5000 or ×12,000; scale bar: 2 μm or 833 nm). (C, D) Immunohistochemical quantification and analysis of ZO-1 and occludin expression (original magnification: ×400; scale bar: 25 μm). Error bars represent SEM. *P < 0.05 vs. ZL rats; #P < 0.05 vs. ZDF rats.