FIGURE 4.

EPO activated the EPOR/JAK2/STAT3 pathway and inhibited NF-κB activation in LPS-induced ALI. EPO (5 U/g) was injected into the peritoneal cavity of mice after LPS (15 mg/kg) stimulation, and the mice were sacrificed 8 h later. The lung tissues were harvested to measure the protein expression levels of p-JAK2, t-JAK2, p-STAT3, t-STAT3, NF-κB p-p65, and NF-κB t-p65 (A–D) by Western blotting. The data are presented as the mean ± SD. The differences among groups were assessed by one-way ANOVA and the post hoc test (Turkey method) was applied to investigate the differences one by one. n = 8 per group.