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. 2020 Mar 19;11:306. doi: 10.3389/fphar.2020.00306

FIGURE 5.

FIGURE 5

The protective effects of EPO on acute lung injury were EPOR/JAK2/STAT3/NF-κB signaling axis dependent. EMP9 (an EPOR antagonist, 1 mg/mouse), Fedratinib (a JAK2 inhibitor, 100 mg/kg), NSC 74859 (a STAT3 inhibitor, 5 mg/kg), BAY 11-7082 (an NF-κB inhibitor, 20 mg/kg), and DMSO (solvent of inhibitors) were injected into the peritoneal cavity 30 min before EPO treatment. Then, EPO (5 U/g) was injected into the peritoneal cavity of mice after LPS (15 mg/kg) stimulation, and the mice were sacrificed 8 h later. The lung tissues were harvested to measure the protein expression of p-JAK2, t-JAK2, p-STAT3, t-STAT3, NF-κB p-p65, and NF-κB t-p65 (A–D) by Western blotting. The data are presented as the mean ± SD. The differences among groups were assessed by one-way ANOVA and the post hoc test (Turkey method) was applied to investigate the differences one by one. n = 8 per group.