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. 2020 Mar 19;13:38. doi: 10.3389/fnmol.2020.00038

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Copyright © 2020 Hornung, Dutta and Bitan.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Electron micrographs of exosomes. Exosomes were isolated from cultured primary endothelial cells. Left: exosomes were stained with uranyl acetate and embedded as whole mount preparations in methylcellulose. The image shows a cup-shaped morphology and heterogeneous sizes ranging from 30 to 100 nm. Right: Exosomes were analyzed by cryoelectron microscopy without chemical fixation or contrasting. Exosomes appear as round membranous structures. Adapted from panels B and C in Figure 1 of Banizs et al., © 2014, originally published in International Journal of Nanomedicine (Dovepress). https://doi.org/10.2147/IJN.S64267.