Figure 3.

Isolation of CNS-derived exosomes from blood. (A) The protocol of the Zhang group relies on anti-L1CAM antibody-coupled epoxy beads, which are incubated directly with diluted plasma to bind neuronal exosomes. The following washing steps in 0.1% BSA remove unbound, non-neuronal exosomes in the sample. (B) The method described by Goetzl et al. The protocol uses first an exosome precipitation step by ExoQuick followed by capturing specifically neuronal exosomes with biotinylated anti-L1CAM antibodies and a streptavidin-conjugated resin. Subsequent washing steps remove non-neuronal exosomes as well as the antibody and resin to yield neuronal exosomes.