Figure 3.

Stimulative capacity of composite nanofibrous mats. hMSCs were cultured on plastic and composite nanofibres in the absence of soluble chondrogenic factors. Following 14 days culture, qPCR was performed to quantify the level of chondrogenic gene expression—Col2, Agg, and Sox-9—as well as Col1. Gene expression levels were normalized to the expression of the housekeeping gene. GAPDH (shown in dotted line). Graph shows mean ± SE. Samples were compared statistically using two-way ANOVA with Bonferroni post-test. *p < 0.05, **p < 0.01, ***p < 0.001, n = 5.