Principal coordinate analysis (PCoA) of bacterial communities at the genus level by groups and environments. Bidimentional representation of the principal coordinate analysis of bacterial communities’ structure using the Yue and Clayton analysis (A) and membership using the Classic Jaccard analysis (B, C, and D). Samples from these 6 healthy horses and 6 asthmatic horses in 3 different environments (green: pasture, black: good quality had, red: dusty hay). C and D, The groups removed from analysis were greyed out. A, The samples’ structure did not cluster significantly by group or by environment. B, The samples’ membership did not cluster by groups when environments were analyzed separately (circles versus diamonds within the same color) but clustered by environment (pasture versus good hay or dusty hay, AMOVA, P < .001). This clustering was not significant when only healthy horses were analyzed (C) but was significant in asthmatic horses (D) (pasture versus good hay or dusty hay, AMOVA, P ≤ .001)