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Engineered mutant activity with the novel chitooligosaccharide oxidase assay. Difference in k cat/K m of purified hyperactive mutants using the 4MU‐chitobioside assay. k cat values are reported in units of 1/s. K m values are reported in units % w/v. Error bars denote propagated SD of fit (accounting for covariance). The A239T/L364Q mutant had too little total activity to measure k cat or K m
