Table 3.

Calculated rate constants of engineered mutants with k _cat values are reported in units of 1/s

	k cat	Fold change (p value)	K m	Fold change (p value)
WT	0.78 ± 0.05	N/A	0.030 ± 0.002	N/A
A239T/L364Q	N.D.	N/A	N.D.	N/A
V246A	0.80 ± 0.07	1.03 (p = 0.7)	0.035 ± 0.004	1.17 (p = 0.1)

Note: K _m values are reported in units % w/v. Fold changes are relative to wild type enzyme.