Figure 5.

LLhF‐DNA binding assayed with filter binding. The filter binding assay was used to monitor LLhF binding to (a) lacO ¹, (b) lacO ², and (c) lacO ³. In these experiments, DNA concentration was fixed at 3 × 10 ⁻¹² M. Only partial binding curves could be obtained for lacO ³ due to limitations on the LLhF concentration that could be obtained from a single protein preparation; this partial curve was used to determine a lower limit for K _d. The curves shown are representative of at least three replicates with at least two different protein preparations. The solid lines are the best fit of Equation (1) to the data