Figure 2. Effects of the DCSIGN1-336 polymorphism on transcriptional activity.

(a) EMSAs with ³²P-labeled probes containing the −336G variant, the −336A variant, the Sp1 consensus site or the AP2 consensus site and HeLa cell nuclear extract. Competition experiments were done in the presence of a tenfold excess of unlabeled Sp1 or AP2 probe. (b) Quantification of the major oligonucleotide-nuclear protein complex (filled arrowhead in panel a). Competition with unlabeled Sp1 consensus probe showed a threefold decrease in binding to the −336G probe, but no significant decrease in binding to the −336A probe. The AP2 consensus probe showed slight and similar competition to both probes. (c,d) Luciferase expression of reporter gene constructs expressing sequence from −472 to −1 in transfected HeLa cells. (c) Schematic representation of reporter gene constructs containing the CD209 promoter region with the DCSIGN1-336 polymorphism. (d) Relative luciferase expression from pGL3-Basic, the parental vector without promoter. Luciferase expression from the CD209 reporters relative to this value is shown.