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. 2005 Dec 20;38(1):38–46. doi: 10.1038/ng1698

Figure 2. L-SIGN is a binding receptor for SARS-CoV and mediates proteasome-dependent viral degradation.

Figure 2

(a) Viral ORF-1b copy number of N7, N5 and N7/5 L-SIGN transfected CHO cells (L-SIGN/CHO), and mock transfectants (CHO), pre-treated with anti-L-SIGN antibodies (clones 120526 and 120612, 10 μg/ml each) or isotype control antibody before being pulsed with 1 PFU/cell SARS-CoV and incubated for 1 h at 4 °C and washed. (b) Total viral ORF-1b copy number (cell lysates plus supernatants) of N7, N5 and N7/5 L-SIGN transfected CHO cells, and mock transfectants, pulsed with 1 PFU/cell SARS CoV for 1h, washed and subsequently incubated at 37 °C for 24 and 48h. (c) Total viral ORF-1b copy number of N7 L-SIGN transfected CHO cells, and mock transfectants, pulsed with 1 PFU/cell SARS CoV for 1 h at 37 °C, washed and subsequently incubated for 4 h in the presence or absence of 10 μM MG132 or proteasome inhibitor I (Pro. Inh. 1). *P < 0.05 in comparison to cells treated with DMSO solvent control. Data are expressed as mean ± s.d. from triplicate, and are representative of 3 experiments.