Figure 5. Homozygous, but not heterozygous, L-SIGN reduced the final total viral genomic and subgenomic copy number in a closed infection system.

(a) 1 × 10⁴ Vero E6 cells were cultured with 1 × 10⁴ (ratio 1:1) or 1 × 10³ (ratio 1:0.1) stable transfectants of N5 or N7/5 L-SIGN transfected CHO cells, and subsequently infected with 0.01 PFU per Vero E6 cell. Without washing, cells were further incubated for 48 h before the determination of the total viral genomic ORF-1b copy number. (b) Cells were treated as in a for the determination of the total nucleocapsid copy number. Data are expressed as mean ± s.d. from triplicate and are representative of three experiments. ^*P < 0.05, ^**P < 0.01 in comparison to heterozygous N7/5 L-SIGN/CHO.