Fig. 1.

Schematics of E93-mediated enhancer regulation and experimental design. (A) Illustrations of larval wing imaginal discs and late pupal wings showing that E93-dependent changes in chromatin accessibility correlate with temporal changes in the activity of two target enhancers (expression patterns indicated in green and red). (B) Schematic of E93 induction and relative timing of precocious E93 expression (teal) versus endogenous E93 expression (magenta). GAL4 drivers in combination with GAL80^ts were used to initiate precocious E93 expression in mid-third instar larvae (L3) for subsequent dissection in wandering third instar larvae (3LW).