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The sts1-DD mutant induces 26S proteasome mislocalization in vivo. Microscopy was performed as in Fig. 5A. Yeast was transformed with either empty vector (p414GPD) or p414GPD-sts1-DD. Lid and base were examined in panel A, while lid and CP were imaged in panel B. Two-ANOVA was used to determine statistical significance of differences (****P<0.0001; ns, not significant). Scale bars: 5 μm. Strains used were MHY6966, MHY6964, MHY6956 and MHY6954.
