Fig. 3.

Three adjacent PDZ domains of Scribble are required to bind HCV NS4B. a Diagram showing the structure of Scribble protein. The Myc-tagged PDZ domains of Scribble used in the Co-IP assay were indicated, and the binding activities of Scribble PDZ domains for HCV NS4B were summarized. b–j 293T cells were transfected with pFLAG-CMV2 or pFLAG-CMV2-NS4B and Myc-tagged Scribble PDZ (b), PDZ1 (c), PDZ2 (d), PDZ3 (e), PDZ4 (f), PDZ1-2 (g), PDZ2-3 (h), PDZ3-4 (i), and PDZ2-4 (j). Cell lysates were mixed with anti-FLAG resin, and the precipitates were analyzed by Western blot with indicated antibodies. k 293T cells were co-transfected with pFLAG-CMV2-NS4B, pCDNA4b, or Myc-tagged Scribble PDZ1-3. Cell lysates were mixed with anti-Myc resin, and the precipitates were analyzed by Western blot with indicated antibodies