Supplementary Table S9.
Flow Cytometry Antibodies
| Target | Dilution | Cat number | Fluorochrome | Supplier |
|---|---|---|---|---|
| CD16 | 1:20 | 48-0168-42 | Efluor450 | ThermoFisher |
| 1:22 | 130-098-101 | APC | Miltenyi Biotec | |
| CD56 | 1:33 | 318316 | Alexa Fluor 700 | BioLegend |
| CD19 | 1:20 | 302242 | BV510 | BioLegend |
| 1:22 | 555412 | FITC | BD | |
| CD20 | 1:33 | 130-096-649 | PE-Cy7 | Miltenyi Biotec |
| CD14 | 1:20 | 555398 | PE | BD |
| CD3 | 1:33 | 317306 | FITC | BioLegend |
| 1:22 | 130-098-162 | FITC | Miltenyi Biotec | |
| CD127 | 1:20 | 351320 | PE-Cy7 | BioLegend |
| HLA-DR | 1:33 | 307636 | BV421 | BioLegend |
| CD11c | 1:20 | 301638 | BV650 | BioLegend |
| CD123 | 1:30 | 306030 | BV711 | BioLegend |
| Lineage markera | 1:10 | B29559 | PE | Beckman Coulter |
| CD15 | 1:33 | 301904 | FITC | BioLegend |
| 1:22 | 130-098-010 | PE | Miltenyi Biotec | |
| IL36R | 1:10 | BAF | Streptavidin | BD |
| Streptavidinb | 1:100 | 405207 | APC | BioLegend |
| CD45 | 1:22 | 130-098-139 | VioBlue | Miltenyi Biotec |
| CD24 | 1:22 | 130-099-935 | APC Vio770 | Miltenyi Biotec |
| PLSCR1 | 1:50 | ab180518 | Rabbit-IgG | Abcam |
| Rabbit IgGc | 1:100 | 406416 | Alexa Fluor 488 | BioLegend |
| IFN-α | 1:10 | 130-092-602 | APC | Miltenyi Biotec |
a
CD3/CD14/CD19/CD20/CD56.
b
Target of secondary biotinylated antibody used for IL36R detection.
c
Target of secondary antibody used for PLSCR1 detection.