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. 2020 Mar 12;16(9):1616–1628. doi: 10.7150/ijbs.40896

Figure 4.

Figure 4

Detectability of indocyanine green (ICG) and Angiostamp800 (A800) by fluorescence and photoacoustic imaging. A) Absorption and fluorescence spectra of ICG (orange) and Angiostamp800 (green). B) Fluorescence signal of various concentrations (n=3 samples/concentration) of ICG, C) Angiostamp800 and fluorescence background noise (BKG) measured by the Fluobeam®800 (excitation 780 nm; emission > 830 nm). The sensitivity threshold (SThr) corresponds to the concentration that provides a signal-to-noise ratio (contrast agent versus PBS) of 1.5. D) Photoacoustic spectra of ICG (orange), Angiostamp800 (green), oxyhemoglobin (dark red) and deoxyhemoglobin (dark blue). E) Photoacoustic signal of various concentrations (n=3 samples/concentration) of ICG, F) Angiostamp800 and photoacoustic background noise (BKG) measured by spectral unmixing analyses from spectroscopic acquisition (680-970 nm). The sensitivity threshold (SThr) corresponds to the concentration that provides a signal-to-noise ratio (contrast agent versus PBS) of 1.5.