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. 2020 Mar 12;16(9):1616–1628. doi: 10.7150/ijbs.40896

Figure 5.

Figure 5

Evaluation of ICG blood pharmacokinetics by real-time noninvasive photoacoustic imaging and ex vivo fluorescence imaging of plasma samples. A) Photoacoustic imaging of the carotid artery (dotted line; longitudinal section) before ICG injection. B) Real-time in vivo monitoring of ICG photoacoustic signal (800 nm) from the carotid artery of three healthy mice after intravenous injection of ICG and non-linear fits of data. C) Ex vivo fluorescence imaging of plasma samples collected at different time points after intravenous injection of ICG. D) Non-linear fits of ex vivo fluorescence signal of plasma samples from three healthy mice after intravenous injection of ICG. E) ICG blood half-lives calculated either from real-time in vivo monitoring of ICG photoacoustic signal from the carotid artery or from ex vivo fluorescence signal of plasma samples collected from mice at various stages of liver metastasis development. n= 3 mice (healthy group), n= 6 mice per group (Early and Advanced); statistical analyses: non-linear fitting curves (one-phase decay) and two-way ANOVA tests were used (*p-value<0.05; **p-value<0.01; ***p-value<0.001; ****p-value<0.0001).