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. 2020 Mar 12;16(9):1629–1639. doi: 10.7150/ijbs.41867

Figure 2.

Figure 2

F2r knockdown promotes osteoclastogenesis. (A) TRAP stain and (B) Acridine orange (AO) stain of sh-sc and sh-F2r infected MBMs that induced 5 days by M-CSF and RANKL in 12-well-plate. (C) Wheat germ agglutinin (WGA) stain of osteoclasts on bone slices to detect bone resorption area. (D) Quantification data of A-C. TRAP-positive MNCs (multinucleated cells, ≥3 nuclei). (E) Western blot of F2r and Ctsk protein expression level in sh-sc and sh-F2r infected MBMs that induced 5 days by M-CSF and RANKL. (F) Quantification data of E by image J. Tubulin was used as a control. Protein expression level in the mock group was normalized as 1. Results are presented as mean ± SEM; one dot represents one sample. n≥3. **p<0.01, ***p<0.001.