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. 2020 Mar 12;16(9):1629–1639. doi: 10.7150/ijbs.41867

Figure 4.

Figure 4

F2r inhibited F-actin ring formation and Ctsk expression in mature osteoclasts on bone slides. (A) Fluorescence microscopy of F-actin ring stain and anti-Ctsk immunofluorescence (IF) stain in mature osteoclasts from MBMs infected by sh-sc and sh-F2r on bone slides. Overlap detected as a yellow-orange area in the merged image. (B) Quantification data of A. (C) F-actin ring stain and anti-Ctsk IF stain for pLX304 and pLX304-F2r infected MBMs that were induced to mature osteoclasts on bone slides. (D) Quantification data of C. Results are presented as mean ± SEM; n≥4. *p<0.05, **p<0.01, ***p<0.001.