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. 2020 Mar 12;16(9):1629–1639. doi: 10.7150/ijbs.41867

Figure 5.

Figure 5

F2r answered RANKL -induced MBMs through Akt and NFκB signaling pathways. (A) Western blot analysis of Pu.1 and NFATc1 expression in osteoclasts at day 5 from MBMs infected by shRNA. Tubulin served as a control. (B) Quantification of A. Mock group protein expression normalized as 1. (C) RT-qPCR was used to detect F2r, Ctsk, Atp6i and Nfatc1 mRNA levels relative to Gapdh in M-CSF and RANKL-induced MBMs. (D) Western blot analysis to detect Akt, p65, IKBα and Erk phosphorylation induced by RANKL in monocytes/macrophages infected sh-sc and sh-F2r-2. (E) Quantification of phospho-protein level versus total protein level by RANKL stimulation. One point represents one sample. Results are presented as mean ± SEM; n>3. *p<0.05, **p<0.01, ***p<0.001. ns, no significant difference.