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. 2020 Mar 5;16(9):1507–1525. doi: 10.7150/ijbs.41798

Figure 8.

Figure 8

(A) Ingenuity Pathway Analysis (IPA) displaying the 13 distinctive hits from the total results of the yeast two-hybrid screen for proteins that interacted with NLRP12. The figure only shows the NLRP12-interacting proteins with hits that have a frequency larger than 2. The solid lines indicate interacting proteins found through our yeast two-hybrid screen. The dashed lines are the NLRP12-interacting proteins previously reported in published data. The purple-colored molecules are those associated with AML. (B) To validate an interaction shown in the IPA diagram, the Myc and DDK tagged form of tumor necrosis factor receptor-associated factor 3 interacting protein 3 (TRAF3IP3) and NLRP12-V5 were co-immunoprecipitated from co-transfected 293T cells. A non-denaturing cell lysate buffer (see Material and Methods) was used to lyse the cell, and co-immunoprecipitations were performed with a mouse monoclonal anti-TRAF3IP3 antibody followed by protein A/G agarose. The immunoprecipitates were analyzed for NLRP12 by immunoblotting with rabbit monoclonal anti-V5 antibody. The results from the co-immunoprecipitation are consistent with a confirmation of an interaction between TRAF3IP3 and NLRP12.