Table 3.

Primers used in cloning (The underline shows the restriction enzymes).

Name	Primers
NLRP12 (a.a. 1-531) 5' primer (cloned into PGBKT7)	GATCTACATATGCTACGAACCGCAGGCAG
NLRP12 (a.a. 1-531) 3' primer (cloned into PGBKT7)	GATGTAGTCGACTCACCCCTCGTCCAGGATATAGTACATAGCT
NLRP3 (a.a. 1-536) 5' primer (cloned into pGBKT7)	GATCTACATATGAAGATGGCAAGCACCCGCTGCA
NLRP3 (a.a. 1-536) 3' primer (cloned into PGBKT7)	GATGTAGGATCCTCACAGCAGGTAGTACATGGCGGCAAAGA
NLRP8 (a.a. 1-527) 5' primer (cloned into pGBKT7)	GATCTAGAATTCAGTGACGTGAATCCACCCTCTG
NLRP8 (a.a. 1-527) 3' primer (cloned into PGBKT7)	GATGTAGGATCCTCAGAGTCTTTGTGGGAAACAGAGAACA
NLRP12 5' (cloned into pEFIRES-P-3FLAG and pIRES-V5)	GTCCAGCTTAAGGCCACCATGCTACGAACCGCAGGCAG
NLRP12 3' (cloned into PIRES-3FLAG and pEFIRES-P-V5)	CTGAATTCTAGAGCAGCCAATGTCCAAATAAGG
HCK p61 5' (cloned into pEFIRES-P-3FLAG)	GATCTACTTAAGGCCACCATGGGGGGGCGCTCAAGCTGCGAGG
HCK p61/p59 3' (cloned into pEFIRES-P-3FLAG)	GTCGATTCTAGATGGCTGCTGTTGGTACTGGCTCTCT