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. 2020 Mar 5;11(11):3246–3255. doi: 10.7150/jca.40953

Figure 2.

Figure 2

Expression of JAM-B in pancreatic cancer (PanCa) cell lines, and the effect of the silencing JAM-B on cell signal pathway. (A)Western blot was used to detect the protein level of JAM-B and JAM-C in 5 PanCa (BxPC-3, Panc-1, AsPC-1, SW1990 and CFPAC-1 cell lines. (B) Western blot analysis with IMAGE J was used to detect the protein level of JAM-B and JAM-C in 5 PanCa cells. The expression of each protein in cells was determined following normalization with a loading control, GAPDH. (C) Transfection efficiency of JAM-B in PanCa cell lines following transfection with JAM-B shRNA at the protein level determined by western blot analysis and the downstream pathway was also analyzed in BxPC-3 and Panc-1. (D and E) Western blot analysis with IMAGE J was used to detect the protein level of JAM-B and the downstream pathway. The expression of each protein in cells was determined following normalization with a loading control, GAPDH. Relative protein levels of JAM-B are shown as bar diagrams (*P<0.05, compared with control and NSC).