Figure 1.

PLK1 was over-expressed and inhibition of PLK1 by BI 2536 reduced viability in neuroblastoma cell lines. (A) Quantification of PLK1 mRNA expression of neuroblastoma cell lines. (B) Western blot analysis of PLK1 expression in neuroblastoma cell lines. (C) Overall survival and event free survival plot generated from SEQC-498 cohorts in R2: Genomics Analysis and Visualization Platform (http://r2.amc.nl). (D) Overall survival and event free survival plot generated from Versteeg-88 cohorts in R2: Genomics Analysis and Visualization Platform (http://r2.amc.nl). (E) Molecular structure of BI 2536 and IC50 value of BI 2536 in neuroblastoma cell lines. The IC50 values were derived after plotting proliferation values on a logarithmic curve. Experiments were performed in quadruplicate and repeated twice. (F) Proliferation rate of neuroblastoma cell lines treated with BI 2536. NB cells (2 × 10⁴) were seeded in 96-well plates overnight and incubated with DMSO or increasing concentrations of BI 2536 (1, 2.5, 5, 10, 25, 50 or 100nM) for 24 h. Cell proliferation rate was calculated as a percentage of the DMSO treated control wells.