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. 2020 Mar 5;11(11):3274–3287. doi: 10.7150/jca.33110

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BI 2536 induced cell cycle arrest in neuroblastoma cells. (A) Photographs of SH-SY5Y and SK-N-BE(2) cells incubated with DMSO or BI 2536 for 24h. Scale bar represents 100 μm. (B) Cell cycle analysis showed SH-SY5Y and SK-N-BE(2) cells displayed cell cycle disorder after treatment with BI 2536. SH-SY5Y cells were harvested after 24h of treatment with 5 or 10nM BI 2536. SK-N-BE(2) cells are treated for 24 h with BI 2536 at 10 or 50nM compared with DMSO control mock treatment. (C) Proportion of the G2 phase cells increased significantly in both SH-SY5Y and SK-N-BE(2) cells after treated with indicated concentration of BI 2536. (D) Confocal analysis of GFP- Histone localization in SH-SY5Y cells. SH-SY5Y was infected by the GFP- Histone lentivirus at the multiplicity of infection (MOI) of 20 for 24h. After media replacement, cells were allowed to grow for another 48h. Then, BI 2536 was added into the cells for 24h before the confocal analysis. Scale bar represents 20 µm.