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. 2020 Mar 5;11(11):3274–3287. doi: 10.7150/jca.33110

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BI 2536 affected the expression of apoptosis-related genes in neuroblastoma cells. (A) Caspase-3 activity assay indicated BI 2536 treatment significantly increased Caspase-3 activity in both SH-SY5Y (left) and SK-N-BE(2) (right) cells. (B) CCK8 assay showed Z-VAD-FMK (25μM) partially reversed BI 2536-induced cell death in SH-SY5Y (left) and SK-N-BE(2) (right) cells. (C) Gene expression clustering of 370 key apoptosis-related genes in 10nM BI 2536-treated SH-SY5Y cells compared to DMSO-treated cells. (D) Transcript level of top 10 up regulated genes in SH-SY5Y cells treated with 10nM BI 2536. (E) Transcript level of top 10 down regulated genes in SH-SY5Y cells treated with 10nM BI 2536. *P<0.05 and ***P < 0.001. P values were determined by two-tailed t tests. All data are representative of three independent experiments with n = 3-6 per group and are means ± s.e.m.