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BI 2536 mitigated autophagy by inactivating AMPK signaling. (A) Western blot analysis of LC3-II, SQSTM1/p62 protein level in SH-SY5Y (left) and SK-N-BE(2) (right) cells after 24hr treatment with serial concentrations of BI 2536. (B) Western blot analysis of phosphorylation of Thr172 AMPKα in SH-SY5Y (left) and SK-N-BE(2) (right) cells incubated with BI 2536 for 24hr.
