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Bufalin inhibited invasion and migration of GBC-SD cells. (A, B) Transwell migration assay of the effects of Bufalin on migration and invasion of GBC-SD cells under different concentrations (0, 25, 50 nM, respectively) (200 ×). (C, D) Western blot analyzed the expression of invasion related proteins MMP2, MMP9, E-cadherin and Snail after 48 hours of treatment by Bufalin at different concentrations. GAPDH was the loading control (*P<0.05 vs Control).
