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Bufalin inhibited the activation of c-MET to prohibit the development of gallbladder cancer. (A) Western blot analyzed the expression of p-c-MET and p-EGFR in GBC-SD cells after 48 hours of treatment by Bufalin and HGF or Bufalin and EGF combinations. (B) Western blot analyzed the expression of proteins associated with invasion, cell cycle and stemness in GBC-SD cells after 48 hours of treatment by Bufalin and HGF. GAPDH was used as the loading control.
