Figure 6.

Seeded aggregation kinetics of the Ure2 prion domain. The aggregation of the Ure2 prion domain was followed with thioflavin T fluorescence at 37 °C without agitation. Four repeats of Ure2 in the absence of fibril seeds (red traces) and in the presence of 10% pre-formed fibril seeds (blue traces) are shown. Note that Ure2 fibril seeds caused a small but notable leftward shift of the aggregation curve, suggesting that fibril seeds promoted Ure2 fibril formation. AU, arbitrary units.