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. 2020 Mar 25;40(3):BSR20192770. doi: 10.1042/BSR20192770

Table 1. Extracellular DNA in blood products and their concentration.

Reference Blood products DNA concentration Sample number DNA type Quantitative method
Duxbury et al., 1995 [11] Whole blood Range 260–1474 ng/ml 7 total DNA Threshold total DNA Assay system
Dijkstra-Tiekstra et al., 2004 [12] PCs (before filtration) 1.7 ±  0.8 leucocyte-eq/μl 5 nucDNA Quantitative real-time polymerase chain reaction
PCs (after fitration) 1.5±   0.8 leucocyte-eq/μl 5
Ivancic-Jelecki et al., 2009 [13] Plasma Range 0.06–22.5 ng/ml 10 nucDNA Quantitative real-time polymerase chain reaction
Lee et al., 2014 [14] RBCUs (LR) Range 0.8–87-fold 11 mitDNA Quantitative real-time polymerase chain reaction
PCs Range 0.4–235.6-fold 5
FFP Range 0.7–46.5-fold 16
Cognasse et al., 2016 [15] AE-associated PCs 484 ±  313.45 ng/ml (peak concentration) 42 mitDNA Quantitative real-time polymerase chain reaction
Control PCs 122.55 ±  52.64 ng/ml (peak concentration) 59
Shih et al., 2016 [16] RBCUs (WBF) 1.08 ±  0.90 ng/ml 44 total DNA PicoGreen assay
RBCUs (WBF) 3.57 ±  1.99 μg/ml 47 Spectrophotometry
RBCUs (RCF) 0.50  ±  0.77 ng/ml 73 PicoGreen assay
RBCUs (RCF) 3.28 ± 1.28 μg/ml 73 Spectrophotometry
Bakkour et al., 2016 [17] RBCUs (non-LR) 5.3 × 105 copies/μl (mean concentration) 12 mitDNA Quantitative real-time polymerase chain reaction
RBCUs (MCS+ apheresis) 1.3 × 105 copies/μl (mean concentration) 12
RBCUs (Trima apheresis) 1.2 × 105 copies/μl (mean concentration) 12
Yashui et al., 2016 [18] NHTR-associated PCs Range 1.4–110 × 104 copies/ml 17 mitDNA Quantitative real-time polymerase chain reaction
NHTR-associated RBCUs Range 0.3–23 × 104 copies/ml 20
NHTR-associated FFP Range 2.3–18 × 104 copies/ml 11
Control PCs Range 0.1–3.8 × 104 copies/ml 320
Simmons et al., 2017 [19] RBCUs 3 ± 0.4 ng/ml 114 mitDNA Quantitative polymerase chain reaction
PCs 94.8 ± 69.2 ng/ml 17
FFP 213.7 ± 65 ng/ml 81
Waldvogel Abramowski et al., 2018 [20] RBCUs 290  ± 120 ng/ml 23 total DNA Qubit Fluorometer
PCs 339.6 ± 114 ng/ml 23
FFP 2.875 ± 0.996 ng/ml 23