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. 2020 Mar 25;40(3):BSR20192620. doi: 10.1042/BSR20192620

Figure 4. Inflammation factor, matrix protease and relative protein of autophagy were regulated by PAR2 in chondrocytes induced by IL-1β.

Figure 4

(A and B) Inflammation factor (Cox-2 and TNF-α) were determined using ELISA. (C and D) matrix protease (MMP-1 and MMP-13) were detected by Western blot and quantification analysis. (E and F) Relative protein of autophagy (LC3II and p62) were detected by Western blot and quantification analysis. (G–J) The expression of LC3II and p62 were further confirmed by immunohistochemistry and quantification analysis. The values are mean ± SD of three independent experiments. **P<0.01 vs NC group, ##P<0.01 vs IL-1β treatment group.