Table 2.
Antibodies used for immunoblotting
| Marker (species) | Dilution | Distributor/source (catalog number) |
|---|---|---|
| Primary antibody | ||
| RANKL mouse mAb | 1:100 | Novus Biologicals (12A380) |
| OSX rabbit mAb | 1:70(IHF) | Santa Cruz (A13) |
| OSX rabbit mAb | 1:1000(WB) | Abcam (ab22552) |
| OPN goat mAb | 1:200(IHF) | R&D (af808) |
| OPN rabbit pAb | 1:1000(WB) | ZEN BIO(380437) |
| COL1A1 goat pAb | 1:200 | Santa Cruz (sc-8784-R) |
| CD31 rabbit mAb | 1:200 | Biolegend (102502) |
| SOX9 rabbit mAb | 1:300 | Millipore (ab-5535) |
| Ror2 rabbit pAb | 1:1000 | CST (4105) |
| Stat3 mouse mAb | 1:1000(WB) | CST (9139) |
| Stat3 mouse mAb | 1:100(ICF) | CST (9139) |
| Runx2 rabbit mAb | 1:1000 | CST (8486) |
| pStat3 mouse mAb | 1:2000 | CST (4113) |
| Secondary antibody | ||
| Anti-rabbit IgG (Fluor® 488 Conjugate) | 1:500 (IHF) | Yeasen Biotech (33106ES60) |
| Anti-mouse IgG (Fluor® 568 Conjugate) | 1:500 (IHF) | Abcam (ab175473) |
| Anti-rat IgG (Fluor® 594 Conjugate) | 1:500 (IHF) | Yeasen Biotech (34412ES60) |
| Anti-mouse IgG (Fluor® 564 Conjugate) | 1:300 (ICF) | Yeasen Biotech (33212ES60) |
| Anti-mouse IgG HRP-linked Ab | 1:2000 (WB) | CST (7076) |
| Anti-rabbit IgG HRP-linked Ab | 1:2000 (WB) | CST (7074) |
IHF immunohistofluorescence, WB western blot, ICF immunocytofluorescence