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. 2020 Mar 26;11:136. doi: 10.1186/s13287-020-01637-3

Fig. 3.

Fig. 3

Anti-apoptotic and anti-anoxia effect of CS-IGF-1C hydrogel and hP-MSC co-transplantation on NMVCs. a Calcein-AM/PI staining was used to assess the influence of CS-IGF-1C hydrogel and hP-MSC co-transplantation on anti-apoptotic effect in NMVCs. b Quantification analysis of Calcein-AM/PI staining. *P < 0.05 versus PBS + H2O2; **P < 0.01 versus PBS + H2O2; #P < 0.05 versus PBS + CS + H2O2. c Anti-anoxia effect was evaluated by Calcein-AM/PI staining. d Quantification analysis results of Calcein-AM/PI staining revealed that the percentage of myocardial cell death were markedly lower in the presence of CS-IGF-1C hydrogel. **P < 0.01 versus Hypoxia; #P < 0.05 versus Hypoxia/PMSCs/CS. e CCK-8 results shows that NMVC survival was ameliorated in response to H2O2 after CS-IGF-1C hydrogel and hP-MSC co-transplantation. *P < 0.05 versus Normal. f Real-time quantitative PCR analysis of Bad gene expression of NMVCs after treated with H2O2. All data were shown as means ± SEM in triplicate assays. *P < 0.05 versus PBS; **P < 0.01 versus PBS; #P < 0.05 versus PMSCs