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. 2020 Mar 19;8:68. doi: 10.3389/fcell.2020.00068

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Copyright © 2020 Sumbal, Chiche, Charifou, Koledova and Li.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Morphology of organoids undergoing long-term lactation. (A) Scheme depicting experimental design. FGF2, FGF2 medium; LM, lactation medium; BOM, basal organoid medium. (B) Bright-field images from time-lapse imaging of organoid morphogenesis under continuous LM treatment (FGF2-LM) or under LM withdrawal and replacement with BOM (FGF2-LM-BOM). Scale bars represent 100 μm. (C,D) Morphometric analysis of organoid area (C) and density (D) from the time-lapse experiment. The plots show mean + SD; n = 2, N = 20 organoids per condition. Two-way ANOVA, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. (E) Hematoxylin and eosin staining of organoids at different time points of long-term lactation. Scale bars represent 100 μm.