Figure 1.

Pine wood smoke particulate matter (WSPM) induced MUC5AC mRNA expression. mRNA expression of the secreted gel-forming mucins, (A) MUC5AC and (B) MUC5B by human lobar bronchial epithelial cells (HLBECs) treated with either vehicle or size-fractionated pine WSPM (20 μg/cm²) for 24 h. C, Dose-dependent effect of pine WSPM F7 on MUC5AC expression by HLBECs. D, Muc5ac mRNA expression in larger and smaller airways/parenchyma isolated from 3 C57BL/6 mice following treatment with either saline or pine WSPM. Data are normalized to vehicle or saline treatment (fold change, y-axis) and are presented as the mean ± SEM from n ≥ 3 replicates. *p < .05, **p < .01, and ****p < .0001.