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. 2020 Jan 16;174(2):278–290. doi: 10.1093/toxsci/kfaa006

Figure 3.

Figure 3.

The pine WSPM chemical and TRPA1 agonist coniferaldehyde, and TRPA1 agonist allyl isothiocyanate (AITC), induced MUC5AC expression. A, MUC5AC induction in HLBECs treated with glyoxal, coniferaldehyde, 5-hydroxymethylfurfural (5-HMF), and vanillin at 100 and 250 μM for 24 h. MUC5AC expression for each treatment is represented as fold change over vehicle treatment. B, Secreted MUC5AC protein concentration measured by ELISA in supernatants of HLBECs treated with vehicle or 250 μM coniferaldehyde for 24 h. C, HLBECs treated with the TRPA1 agonist AITC (60 μM) for 24 h show significant upregulation of MUC5AC expression. Data are presented as fold change over vehicle treatment. Data are presented as the mean ± SEM from n ≥ 3 treatments. *p < .05, **p < .01, and ***p < .001.