Figure 2. Generation of Hspa1l^−∕− mice.

(A) Schematic representation of targeting strategy using CRISPR/Cas9 system; (B) Sanger sequencing shows a 125-bp deletion were detected in Hspa1l^−∕− mice; (C) Agarose gel electrophoresis analysis proved the 125-bp deletion in Hspa1l^−∕− mice; (D) Western blot showing no band at expected size (70 kD) in Hspa1l^−∕− testis; (E–F) HSPA1L expression from Hspa1l^+∕+ and Hspa1l^−∕− adult mice by immunofluorescence.